| Background: Tissue engineering is now increasingly focusing on cell-based treatments as promising tools to improve tendon repair. However, many crucial aspects of tendon biology remain to be understood before adopting the best experimental approach for cell-tissue engineering. Methods: The role played by Ascorbic Acid (AA) alone and in combination with thyroid hormone T3 in the viability and proliferation of primary human tendon-derived cells was investigated. Human tenocyte viability was detected by Trypan blue exclusion test and cellular proliferation rate was evaluated by CFSE CellTrace™. In addition, the potential role of the AA in the production of Nitric Oxide (NO) was also examined. Results: In this in vitro model, an increase in tenocyte proliferation rate was observed as a consequence of progressively increased concentrations of AA (from 10 to 50 μg/ml). The addition of the T3 hormone to the culture further increased tenocyte proliferation rate. In detail, the most evident effect on cellular growth was achieved using the combined supplementation of 50 μg/ml AA and 10-7 M T3. Conclusion: We showed that the highest concentration of AA (100 and 500 μg/ml) caused cytotoxicity to human tenocytes. Moreover, it was shown that AA reduces NO synthesis. These results show that AA is a cell proliferation inducer that triggers tenocyte growth, while it reduces NO synthesis. |
“Combined supplementation of ascorbic acid and thyroid hormone T3 affects tenocyte proliferation. The effect of ascorbic acid in the production of nitric oxide”
Di Giacomo V., Berardocco M., Gallorini M., Oliva F., Colosimo A., Cataldi A., Maffulli N., Berardi A.C.
Original Article, 11 - 18
Keywords: ascorbic acid, cell proliferation, human tenocytes, nitric oxide, T3 thyroid hormone, tendon,
Table of Content: Vol. 7 (No. 1) 2017 January/March
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I.S.Mu.L.T. first-time patellar dislocation guidelines
Vetrano M., Oliva F., Bisicchia S., Bossa M., De Carli A., Di Lorenzo L., Erroi D., Forte A., Foti C., Frizziero A., Gasparre G., Giai Via A., Innocenti B., Longo U.G., Mahmoud A., Masiero S., Mazza D., Natali S., Notarangelo C., Osti L., Padulo J., Pellicciari L., Perroni F., Piccirilli E., Ramponi C., Salvatore G., Schiavone Panni A., Suarez T., Tarantino U., Vittadini F., Vulpiani M.C., Ferretti A., Maffulli N.Editorial, 1 - 10 -
“Combined supplementation of ascorbic acid and thyroid hormone T3 affects tenocyte proliferation. The effect of ascorbic acid in the production of nitric oxide”
Di Giacomo V., Berardocco M., Gallorini M., Oliva F., Colosimo A., Cataldi A., Maffulli N., Berardi A.C.Original Article, 11 - 18 -
Arthroscopic trans-osseous rotator cuff repair
Chillemi C., Mantovani M.Technical Note, 19 - 25 -
Computerized tomographic assessment and clinical evaluation in shoulder instability treated with the Latarjet-Patte procedure using one screw and washer
Cautiero F., Russo R., Di Pietto F., Sabino G.Original Article, 26 - 33 -
Pre-operative corticosteroid injections improve functional outcomes in patients undergoing arthroscopic repair of high-grade partial-thickness rotator cuff tears
Donohue N.K., Prisco A.R., Grindel S.I.Original Article, 34 - 39 -
Functional treatment in rotator cuff tears: is it safe and effective? A retrospective comparison with surgical treatment
De Carli A., Fabbri M., Lanzetti R.M., Ciompi A., Gaj E., Beccarini G., Vetrano M., Ferretti A.Original Article, 40 - 45 -
Conservative, minimally invasive and open surgical repair for management of acute ruptures of the Achilles tendon: a clinical and functional retrospective study
Maffulli G., Del Buono A., Richards P., Oliva F., Maffulli N.Original Article, 46 - 52 -
Intratendinous tears of the Achilles tendon – a new pathology? Analysis of a large 4-year cohort
Chan O., Morton S., Pritchard M., Parkes T., Malliaras P., Crisp T., Padhiar N., Maffulli N., King J., Morrisey D.Original Article, 53 - 61 -
Morphometric evaluation of human tendocalcaneus: a cadaveric study of south indian male population
Kumar N., Aithal A.P., Nayak S.B., Patil J-, Padavinangadi A., Ray B.B.Original Article, 10.11138/mltj/2017.7.1.062 -
Activity level recovery after acute Achilles tendon rupture surgically repaired: a series of 29 patients with a mean follow-up of 46 months
Zayni R., Coursier R., Zakaria M., Desrousseaux J-F, Cordonnier D., Polveche G.Original Article, 69 - 77 -
Outcomes of prolotherapy for intra-tendinous Achilles tears: a case series
Chan O., Havard B., Morton S., Pritchard M., Maffulli N., Crisp T., Padhiar N., Perry J.D., King J., Morrissey D.Original Article, 78 - 87 -
Topical polidocanol application in combination with static stretching in tendinopathies: a prospective pilot study
Gatz M., Schrading S., Dirrichs T., Tingart M., Rath B., Kuhl C., Quack V.Original Article, 88 - 97 -
Conservative treatment for Insertional Achilles Tendinopathy: platelet-rich plasma and focused shock waves. A retrospective study
Erroi D., Sigona M., Suarez T., Trischitta D., Pavan A., Vulpiani M.C., Vetrano M.Original Article, 98 - 106 -
Plantar fasciitis in athletes: diagnostic and treatment strategies. A systematic review
Petraglia F., Ramazzina I., Costantino C.Review Article, 107 - 118 -
Comparison of dynamic postural stability scores between athletes with and without chronic ankle instability during lateral jump landing
Shiravi Z., Shadmehr A., Talebian Moghadam S., Moghadam B.A.Original Article, 119 - 124 -
The influence of muscle-tendon forces on ACL loading during jump landing: a systematic review
Oberhofer K., Nasab S.H.H., Schütz P., Postolka B., Snedeker J.G., Taylor W.R., List R.Review Article, 125 - 135 -
Effects of the menstrual cycle on lower-limb biomechanics, neuromuscular control, and anterior cruciate ligament injury risk: a systematic review
Balachandar V., Marciniak J.-L., Wall O., Balachandar C.Review Article, 136 - 146 -
Semitendinosus muscle architecture during maximum isometric contractions in individuals with anterior cruciate ligament reconstruction and controls
Karagiannidis E., Kellis E., Galanis N., Vasilios B.Original Article, 147 - 151 -
Chondrocytes treated with different shock wave devices
Notarnicola A., Iannone F., Maccagnano G., Lacarpia N., Bizzoca R., Natuzzi D., Moretti B.Original Article, 152 - 156 -
Ipsilateral hamstring tendon graft reconstruction for chronic patellar tendon ruptures: surgical technique
Maffulli N., Del Buono A., Oliva F.Original Article, 157 - 162 -
Assessment of different fitting methods for in-vivo bi-component T2* analysis of human patellar tendon in magnetic resonance imaging
Liu F., Kijowski R.Original Article, 163 - 172 -
Muscle fatigue in participants of indoor cycling
de Melo dos Santos R., Costa e Costa F., Sepeda Saraiva T., Callegari BOriginal Article, 173 - 179 -
The influence of playing surface on injury risk in italian elite rugby players
Lanzetti R.M., Lupariello D., Venditto T., Rota P., Guzzini M., Vadalà A., Rota A., Ferretti A.Original Article, 180 - 185 -
Does combined strength training and local vibration improve isometric maximum force? A pilot study
Goebel R., Haddad M., Kleinöder H., Yue Z., Heinen T., Mester J.Original Article, 186 - 191 -
Ultrasound measures of tendon thickness: Intra-rater, Inter-rater and Inter-machine reliability
del Baño-Aledo M.E., Martínez-Payá J.J., Ríos-Díaz J., Mejías-Suárez S., Serrano-Carmona S., de Groot-Ferrando A.Original Article, 192 - 199 -
PTX3: a new mediator of bone metabolism and osteoporosis
Tarantino U., Feola M., Celi M., Scimeca M.Letter to the Editor, 200 - 201