| The platelet-rich plasma (PRP) is an autologous biotherapy commonly used for its healing properties. Once activated, platelets released a real “cocktail” of growth factor and cytokines implied in numerous regenerative processes. However the impact of medical practices associated to PRP therapeutic use on platelets functionality remains poorly known. Objectives: we evaluated the in vitro effects of two commonly used local anesthetics (Xylocaine* and Naropin*) on PRP functionality. We also investigated the quantity and quality of PRP that passed through the smallest gauge needle commercialized. Materials and methods: PRP from 9 healthy volunteers were prepared using our previously described home made purification protocol. Platelet aggregation capacity was evaluated by aggregometry assays and the growth factor release was determined by ELISA after platelet activation. We also evaluated the platelet activation status, reactivity and stability of platelets by flow cytometry using the P-selectin expression marker. Results: the association of local anaesthetics with PRP injections resulted in a significant decrease of platelets functionality, assessed by their capacity of aggregating. Local anaesthetics did not interfere with the growth factor release. The different needle sizes and calibres tested for PRP injections did not influence the platelet functionality. Conclusions: the use of local anaesthetics to prevent pain during PRP injections could compromise the therapeutic potential of PRP. These results suggest using carefully local anaesthetics or limiting their use as often is possible. To minimize injection pain, we recommend using 30 G needles. These data will lead to clinical recommendations for painless and controlled PRP injections. |
Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality
Bausset O., Magalon J., Giraudo L., Loui M.-L., Serratrice N., Frere C., Magalon G., Dignat-George F., Sabatier F.
Original Article, 18 - 23
Keywords: growth factors, Platelet concentrate local anaesthetics, platelet rich plasma, PRP, regenerative medicine, sport medicine, tendinopathy,
Table of Content: Vol. 4 (No. 1) 2014 January/March
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Platelet rich plasma therapies: a great potential to be harnessed
Andia I.Editorial, 1 - 2 -
PRP treatment effects on degenerative tendinopathy – an in vitro model study
Zhang J., Wang J.H.C.Original Article, 10 - 17 -
Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality
Bausset O., Magalon J., Giraudo L., Loui M.-L., Serratrice N., Frere C., Magalon G., Dignat-George F., Sabatier F.Original Article, 18 - 23 -
Sonographic findings during and after Platelet Rich Plasma injections in tendons
Abate M., Verna S., Di Gregorio P., Salini V., Schiavone C.Original Article, 29 - 34 -
Learning about PRP using cell-based models
Beitzel K., McCarthy M.B., Russell R.P., Apostolakos J., Cote M.P., Mazzocca A.D.Review Article, 38 - 45 -
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment
Durant T.J.S., Dyment N., McCarthy M.B.R., Cote M.P., Arciero R.A., Mazzocca A.D., Rowe D.Original Article, 45 - 51 -
Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience
Rubio-Azpeitia E., Andia I.Review Article, 52 - 62 -
Tendon structure, disease, and imaging
Weinreb J.H., Sheth C., Apostolakos J., McCarthy M.B., Barden B., Cote M.P., Mazzocca A.D.Review Article, 66 - 73 -
In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood
Intravia J., Allen D.A., Durant T.J.S., McCarthy M.B.R., Russell R., Beitzel K., Cote M.P., Dias F., Mazzocca A.D.Article, 79 - 84 -
The use of odds ratio in the large population-based studies: Warning to readers
Di Lorenzo L., Coco V., Forte F., Trinchese G.F., Forte A.M., Pappagallo M.Letter to the Editor, 90 - 92 -
Meniscal sutures with outside-in technique: our experience with a less expensive method
Calderazzi F., Violi C., Paraskevopoulos A., Schiavi P., Ceccarelli F.Letter to the Editor, 93 - 94 -
No difference at two years between all inside transtibial technique and traditional transtibial technique in anterior cruciate ligament reconstruction
Volpi P., Balt C., Cervellin M., Denti M., Prospero E., Morenghi E., Quaglia A.Letter to the Editor, 95 - 99