| Purpose. This study investigates the antibacterial properties of two different platelet-rich plasma (PRP) platelet concentration preparations (PRPLP and PRPHP) through a time-kill assay. Methods. Two different PRP preparations – a single spin process yielding lower white blood cells and platelet concentration (PRPLP) and one yielding high platelet and white blood cell concentration (PRPHP) – were obtained from 2 individuals. PRPLP, PRPHP, phosphate buffered saline (PBS), whole blood and Cefazolin were added to experimental reaction tubes, each containing a single bacterial inoculum of Staphylococcus aureus (S.aureus), Staphylococcus epidermidis (S. epi), methicillin-resistant Staphylococcus aureus (MRSA) or Propionibacterium acnes (P. acnes). Two dilutions (1:1,000, and 1:10,000) were plated in duplicate tubes, along with positive (blood and PBS) and negative (Cefazolin) controls and assessed at five time points (0, 1, 4, 8 and 24 hours). Results. After centrifugation, platelet count of PRPLP was 386 ± 65.5 x 103/?L and PRPHP was 867 ± 234.4 x 103/?L. Both PRP products showed a significant decrease (p<0.05) in bacterial growth at 8 hours compared to whole blood. Conclusion. The application of PRPLP and PRPHP showed a significant decrease in bacterial growth after 8 hours for S. aureus, S. epi, MRSA and P. acnes compared to the whole blood control group. S.epi, MRSA, and P. acnes also showed a significant decrease in bacterial growth after 24 hours. Despite differences in platelet concentration and WBC concentration, no difference in antibacterial activity was seen between the two preparations |
In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood
Intravia J., Allen D.A., Durant T.J.S., McCarthy M.B.R., Russell R., Beitzel K., Cote M.P., Dias F., Mazzocca A.D.
Article, 79 - 84
Keywords: "antibacterial, antimicrobial.", platelet rich plasma, PRP,
Table of Content: Vol. 4 (No. 1) 2014 January/March
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Platelet rich plasma therapies: a great potential to be harnessed
Andia I.Editorial, 1 - 2 -
PRP treatment effects on degenerative tendinopathy – an in vitro model study
Zhang J., Wang J.H.C.Original Article, 10 - 17 -
Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality
Bausset O., Magalon J., Giraudo L., Loui M.-L., Serratrice N., Frere C., Magalon G., Dignat-George F., Sabatier F.Original Article, 18 - 23 -
Sonographic findings during and after Platelet Rich Plasma injections in tendons
Abate M., Verna S., Di Gregorio P., Salini V., Schiavone C.Original Article, 29 - 34 -
Learning about PRP using cell-based models
Beitzel K., McCarthy M.B., Russell R.P., Apostolakos J., Cote M.P., Mazzocca A.D.Review Article, 38 - 45 -
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment
Durant T.J.S., Dyment N., McCarthy M.B.R., Cote M.P., Arciero R.A., Mazzocca A.D., Rowe D.Original Article, 45 - 51 -
Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience
Rubio-Azpeitia E., Andia I.Review Article, 52 - 62 -
Tendon structure, disease, and imaging
Weinreb J.H., Sheth C., Apostolakos J., McCarthy M.B., Barden B., Cote M.P., Mazzocca A.D.Review Article, 66 - 73 -
In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood
Intravia J., Allen D.A., Durant T.J.S., McCarthy M.B.R., Russell R., Beitzel K., Cote M.P., Dias F., Mazzocca A.D.Article, 79 - 84 -
The use of odds ratio in the large population-based studies: Warning to readers
Di Lorenzo L., Coco V., Forte F., Trinchese G.F., Forte A.M., Pappagallo M.Letter to the Editor, 90 - 92 -
Meniscal sutures with outside-in technique: our experience with a less expensive method
Calderazzi F., Violi C., Paraskevopoulos A., Schiavi P., Ceccarelli F.Letter to the Editor, 93 - 94 -
No difference at two years between all inside transtibial technique and traditional transtibial technique in anterior cruciate ligament reconstruction
Volpi P., Balt C., Cervellin M., Denti M., Prospero E., Morenghi E., Quaglia A.Letter to the Editor, 95 - 99