|Articular cartilage injuries of the knee are difficult to treat due to the poor healing ability of cartilage and conventional treatment methods often give unsatisfactory results. Mesenchymal Stem Cells (MSCs) have generated interest as an alternative source of cells for cartilage tissue engineering due to their chondrogenic potential and their easy isolation from bone marrow. It has been reported that the use of scaffold in cartilage engineering acts as a support for cell adhesion, keeping the cells in the cartilage defects and therefore facilitating tissue formation, and that Hyaluronic acid (HA) is a molecule of particular interest for producing scaffold for tissue engineering.
In this study we evaluated the in vitro selection and expansion of Bone Marrow MSCs (BM-MSCs) and by residual BM+HA membrane (BM-HA-MSCs) used as scaffold. Sixty mL of BM have been aspirated by the posterior iliac crest and HA membrane (Hyalograft-C, Fidia Advanced Biopolimers) was used as scaffold.
BM-MSCs were cultured with D-MEM supplemented with Desamethasone, Ascorbic Acid, β-Transforming Growth Factor and Insulin. When cultured in chondrogenic selective medium MSCs from both BM and HA membrane were able to differentiate into chondrogenesis, but BM-HA-MSCs showed a higher staining intensity than BM-MSCs when they were stained with Toluidine blue. The interaction of MSCs with the HA-scaffold seems to promote by itself chondrogenesis.
In vitro release and expansion of mesenchymal stem cells by a hyaluronic acid scaffold used in combination with bone marrow
Spoliti M., Iudicone P., Leone R., De Rosa A., Rossetti F.R., Pierelli L.
Original Article, 289 - 294
Keywords: bone marrow, cartilage injuries, mesenchymal stem cells, tissue engineering.,
Table of Content: Vol. 2 (No. 4) 2012 October /December
Heron N., Malliaropoulos N.G.Editorial, 248 - 252
Biz C., Favero L., Stecco C., Aldegheri R.Original Article, 282 - 288
In vitro release and expansion of mesenchymal stem cells by a hyaluronic acid scaffold used in combination with bone marrowSpoliti M., Iudicone P., Leone R., De Rosa A., Rossetti F.R., Pierelli L.Original Article, 289 - 294
Frizziero A., Ferrari R., Giannotti E., Ferroni C., Poli P., Masiero S.Review Article, 295 - 301
Rehabilitation after endoscopically percutaneous intramuscular splintage for vastus medialis partial rupture – A case report and review of the literatureKaya D., Doral M.N., Güney H., Karanfil Y., Yildirim M., Utku B., Alkan E., Akin U.Original Article, 302 - 304
Malliaropoulos N.G.Original Article, 309 - 311