| Introduction: cell-based tissue engineering strategies using human mesenchymal stem cells (hMSCs) may help to augment tendon healing. To further investigate the in-vitro behavior of this cell population, we investigated low oxygen culture levels, and growth factor supplementation and their effect on expression of tendon extracellular proteins and cell proliferation. Materials and methods: bone marrow aspirate (BMA) was harvested during arthroscopic rotator cuff repair. Characterized hMSCs derived from BMA were incorporated into 3-dimensional tissue engineered constructs (TECs). TECs were analyzed by frozen sections with immunohistochemistry for cell density, collagen I and collagen III expression. Results: growth factor stimulation and low oxygen increased cell density within TECs. Low oxygen and addition of growth factors to culture media demonstrated an increase in collagen I and III expression, both in ambient oxygen conditions and low oxygen conditions. Conclusion: low oxygen and TGFβ3 demonstrated a positive effect on cell number, and type I and III collagen expression in 3D culture environments. |
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment
Durant T.J.S., Dyment N., McCarthy M.B.R., Cote M.P., Arciero R.A., Mazzocca A.D., Rowe D.
Original Article, 45 - 51
Keywords: construct, low oxygen, mesenchymal stem cells, tendon, transforming growth factor beta.,
Table of Content: Vol. 4 (No. 1) 2014 January/March
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Platelet rich plasma therapies: a great potential to be harnessed
Andia I.Editorial, 1 - 2 -
PRP treatment effects on degenerative tendinopathy – an in vitro model study
Zhang J., Wang J.H.C.Original Article, 10 - 17 -
Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality
Bausset O., Magalon J., Giraudo L., Loui M.-L., Serratrice N., Frere C., Magalon G., Dignat-George F., Sabatier F.Original Article, 18 - 23 -
Sonographic findings during and after Platelet Rich Plasma injections in tendons
Abate M., Verna S., Di Gregorio P., Salini V., Schiavone C.Original Article, 29 - 34 -
Learning about PRP using cell-based models
Beitzel K., McCarthy M.B., Russell R.P., Apostolakos J., Cote M.P., Mazzocca A.D.Review Article, 38 - 45 -
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment
Durant T.J.S., Dyment N., McCarthy M.B.R., Cote M.P., Arciero R.A., Mazzocca A.D., Rowe D.Original Article, 45 - 51 -
Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience
Rubio-Azpeitia E., Andia I.Review Article, 52 - 62 -
Tendon structure, disease, and imaging
Weinreb J.H., Sheth C., Apostolakos J., McCarthy M.B., Barden B., Cote M.P., Mazzocca A.D.Review Article, 66 - 73 -
In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood
Intravia J., Allen D.A., Durant T.J.S., McCarthy M.B.R., Russell R., Beitzel K., Cote M.P., Dias F., Mazzocca A.D.Article, 79 - 84 -
The use of odds ratio in the large population-based studies: Warning to readers
Di Lorenzo L., Coco V., Forte F., Trinchese G.F., Forte A.M., Pappagallo M.Letter to the Editor, 90 - 92 -
Meniscal sutures with outside-in technique: our experience with a less expensive method
Calderazzi F., Violi C., Paraskevopoulos A., Schiavi P., Ceccarelli F.Letter to the Editor, 93 - 94 -
No difference at two years between all inside transtibial technique and traditional transtibial technique in anterior cruciate ligament reconstruction
Volpi P., Balt C., Cervellin M., Denti M., Prospero E., Morenghi E., Quaglia A.Letter to the Editor, 95 - 99