|Muscle extracellular matrix (ECM) plays an important role in maintaining muscular integrity and force transduction. However, the role of ECM in skeletal muscle atrophy remains unknown. In this study, we employed two clinically relevant mouse models of Achillotenotomy and hindlimb suspension to simulate Achilles tendon rupture and hindlimb disuse. The gastrocnemius was harvested following two weeks of treatment. We hypothesized that degradation of muscle ECM basement membrane lead to dysfunction of muscle contractility. Our results demonstrated a significant reduction of gastrocnemius single twitch force, isometric tetanic force, and contraction velocity following tendon rupture (p<0.001), but not disuse.
Additionally, up-regulation of matrix metalloproteinase-2 (MMP-2) was observed only after tendon rupture (p=0.00234). These findings suggest that ECM remodeling and basement membrane degradation due to MMP-2 may be responsible for declined muscle contractibility. Inhibiting ECM degradation enzymes may be a potential treatment strategy for skeletal muscle atrophy after tendon rupture.
Muscle extracellular matrix degradation and contractibility following tendon rupture and disuse
Zhang Q., Joshi S.K., Manzano G., Lovett D.H., Kim H.T., Liu X.
Original Article, 35 - 41
Keywords: Achillotenotomy, basement membrane, extracellular matrix., hindlimb suspension, matrix metalloproteinase-2, muscle atrophy,
Table of Content: Vol. 3 (No. 1) 2013 January/March
Tresoldi I., Oliva F., Benvenuto M., Fantini M., Masuelli L., Bei R., Modesti A.Original Article, 2 - 6
Docking S., Samiric T., Scase E., Purdam C., Cook J.Original Article, 7 - 11
Birch H.L., Thorpe C.T., Rumian A.P.Review Article, 12 - 22
Vesentini S., Redaelli A., Gautieri A.Original Article, 23 - 24
Zhang Q., Joshi S.K., Manzano G., Lovett D.H., Kim H.T., Liu X.Original Article, 35 - 41
Tendon structure and extracellular matrix components are affected by spasticity in cerebral palsy patientsGagliano N., Menon A., Martinelli C., Pettinari L., Panou A., Milzani A., Dalle-Donne I., Portinaro N.M.Original Article, 42 - 50
Del Buono A., Oliva F., Osti L., Maffulli N.Review Article, 51 - 57