| We characterized the ef fect of ciprof loxacin (CPX) in cultured human tenocytes by morphological and molecular methods. Collagen type I and III mRNA and protein levels were unaffected, but lysyl hydroxylase 2b mRNA levels progressively decreased after CPX administration. MMP-1 protein levels significantly increased after 20 μg/ml CPX administration but remained unmodified at the higher dose, whilst MMP-2 activity was unchanged. Tissue inhibitor of MMP (TIMP-1) gene expression decreased after CPX treatment, whilst TIMP-2 and transforming growth factor-β1 gene expression, the cytoskeleton arrangement, and cytochrome c expression remained unmodified. Secreted Protein Acidic and Rich in Cysteine mRNA and protein levels remained almost unchanged, whilst N-cadherin mRNA levels resulted significantly down-regulated and connexin 43 gene expression tended to decrease after CPX administration. The CPX-induced decreased ability to cross-link collagen and decreased TIMP-1 levels, possibly leading to higher activity of MMPs in ECM degradation, together with the down-regulation of Ncadherin and connexin 43 are consistent with a reduced ability to maintain tissue homeostasis, possibly making the tendon more susceptible to rupture. |
New insights in extracellular matrix remodeling and collagen turnover related pathways in cultured human tenocytes after ciprofloxacin administration
Menon A., Pettinari L., Martinelli C., Colombo G., Portinaro N., Delle-Donne I., D'Agostino M.C., Gagliano N.
Original Article, 122 - 131
Keywords: ciprofloxacin, collagen turnover, extracellular matrix remodelling, tendons, tenocytes,
Table of Content: Vol. 3 (No. 3) 2013 July/September
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Sample size determination for clinical research
Wang D., Bakhai A., Del Buono A., Maffulli N.Original Article, 116 - 117 -
Tendon cell ciliary length as a biomarker of in situ cytoskeletal tensional homeostasis
Lavagnino M., Gardner K., Sedlak A.M., Arnoczky S.POriginal Article, 118 - 121 -
New insights in extracellular matrix remodeling and collagen turnover related pathways in cultured human tenocytes after ciprofloxacin administration
Menon A., Pettinari L., Martinelli C., Colombo G., Portinaro N., Delle-Donne I., D'Agostino M.C., Gagliano N.Original Article, 122 - 131 -
Matrix metalloproteases and their inhibitors are altered in torn rotator cuff tendons, but also in the macroscopically and histologically intact portion of those tendons
Castagna A., Cesari E., Garofalo R., Gigante A., Conti M., Markopoulos N., Maffulli N.Original Article, 132 - 138 -
Augmenting tendon and ligament repair with platelet-rich plasma (PRP)
Yuan T., Zhang C-Q, WangOriginal Article, 139 - 149 -
Instruments and techniques for the analysis of wheelchair propulsion and upper extremity involvement in patients with spinal cord injuries: current concept review
Dellabiancia F., Porcellini G., Merolla G.Review Article, 150 - 156 -
Is there a correlation between biophotonical, biochemical, histological, and visual changes in thecartilage of osteoarthritic knee-joints?
Stumpfe S.T., Pester, Steinert S., Marintschev I., Plettenberg H., Aurich M., Hofmann G.O.Original Article, 157 - 165 -
Semitendinosus snapping: analysis of movement, electromyographic activities, muscle strength and endurance, motor control and joint position sense
Guney H., Kaya D., Yilgor C., Cilli M., Aritan S., Yuksel I., Doral M.N.Original Article, 166 - 172 -
The genetics of sports injuries and athletic performance
Maffulli N., Margiotti K., Longo U.G., Loppini M., Fazio V.M., Denaro V.Review Article, 179 - 189 -
The intercalated BSc in sports and exercise medicine at Barts and The London School of Medicine and Dentistry
Morrisey D., Nutt J.L., Mehdian R., Maffulli N.Review Article, 190 - 195 -
Impingement is not impingement: the case for calling it “Rotator Cuff Disease”
McFarland E.G., Maffulli N., Del Buono A., Murrell G. A. C., Garzon-Muvdi J., Petersen S.A.Original Article, 196 - 200 -
Thyroid hormones and tendon: current views and future perspectives.
Oliva F., Berardi A. C., Misiti S., Maffulli N.Review Article, 201 - 203 -
Comparison of reasorbeable mini anchor versus non reasorbeable: histological evaluation in an experimental rabbit model
Pontini A., Munivrana I., Valente M.L., Bassetto F.Original Article, 204 - 209 -
The insertion of the anterior horn of the medial meniscus: an anatomic study
Brown A. A.Original Article, 210 - 212 -
Arthroscopic repair of the subscapularis tendon: indications, limits and technical features
Osti L., Soldati F., Del Buono A., Buda M.Review Article, 213 - 219 -
Suture techniques for tendon repair; a comparative review
Rawson S., Cartmell S., Wong J.Review Article, 220 - 228 -
Tissue-engineered augmentation of a rotator cuff tendon using a reconstituted collagen scaffold: a histological evaluation in sheep
Van Kampen C., Arnoczky S., Parks P., Hackett E., Ruehlman D., Turner A., Schlegel T.Original Article, 229 - 235 -
Non-Hodgkin’s lymphoma: unexpected cause of shoulder pain. A systematic review of the literature
Caporale M.F., Gambino G.F., Larosa F.S., Del Buono A., Di Segni FCase Report, 236 - 239