| Platelet-rich plasma (PRP) has become a popular option for the treatment of injured tendons. However, the efficacy of PRP treatment is a matter of heated debate in orthopaedics and sports medicine. In this study, we used a cell culture model to evaluate the potential effects of PRP treatment on degenerative tendinopathy. The in vitro model, which uses the current concept of “diseases-in-adish”, consisted of tendon stem/progenitor cells (TSCs) that were derived from rabbit tendons and cultured in differentiating media with and without autologous platelet-rich clot releasate (PRCR). We found that 10% PRCR treatment of TSCs blocked their non-tenogenic differentiation, as evidenced by the marked decrease in lipid droplets, proteoglycan accumulation, and calcium deposition on cell surfaces. Moreover, the protein markers for non-tenocytes (adiponectin, collagen type II, and osteocalcin) were either minimally expressed or greatly reduced. However, after TSCs underwent non-tenogenic differentiation by pre-treatment in non-tenogenic media for two days, PRCR only slightly reduced adipogenesis and osteogenesis of TSCs, although chondrogenesis was markedly suppressed. Finally, PRCR treatment after pretreatment of TSCs in non-tenogenic media for one week had little effect on any of the three nontenogenic differentiations of TSCs. These findings suggest that the injection of PRP in clinics may not be able to effectively reverse the degenerative conditions of late-stage tendinopathy, which are characterized by lipid depositions, proteoglycan accumulation, and calcification, either alone or in combination. |
PRP treatment effects on degenerative tendinopathy – an in vitro model study
Zhang J., Wang J.H.C.
Original Article, 10 - 17
Keywords: degenerative tendinopathy, disease-in-a-dish-model., PRP, tendon stem cells,
Table of Content: Vol. 4 (No. 1) 2014 January/March
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Platelet rich plasma therapies: a great potential to be harnessed
Andia I.Editorial, 1 - 2 -
PRP treatment effects on degenerative tendinopathy – an in vitro model study
Zhang J., Wang J.H.C.Original Article, 10 - 17 -
Impact of local anaesthetics and needle calibres used for painless PRP injections on platelet functionality
Bausset O., Magalon J., Giraudo L., Loui M.-L., Serratrice N., Frere C., Magalon G., Dignat-George F., Sabatier F.Original Article, 18 - 23 -
Sonographic findings during and after Platelet Rich Plasma injections in tendons
Abate M., Verna S., Di Gregorio P., Salini V., Schiavone C.Original Article, 29 - 34 -
Learning about PRP using cell-based models
Beitzel K., McCarthy M.B., Russell R.P., Apostolakos J., Cote M.P., Mazzocca A.D.Review Article, 38 - 45 -
Mesenchymal stem cell response to growth factor treatment and low oxygen tension in 3-dimensional construct environment
Durant T.J.S., Dyment N., McCarthy M.B.R., Cote M.P., Arciero R.A., Mazzocca A.D., Rowe D.Original Article, 45 - 51 -
Partnership between platelet-rich plasma and mesenchymal stem cells: in vitro experience
Rubio-Azpeitia E., Andia I.Review Article, 52 - 62 -
Tendon structure, disease, and imaging
Weinreb J.H., Sheth C., Apostolakos J., McCarthy M.B., Barden B., Cote M.P., Mazzocca A.D.Review Article, 66 - 73 -
In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood
Intravia J., Allen D.A., Durant T.J.S., McCarthy M.B.R., Russell R., Beitzel K., Cote M.P., Dias F., Mazzocca A.D.Article, 79 - 84 -
The use of odds ratio in the large population-based studies: Warning to readers
Di Lorenzo L., Coco V., Forte F., Trinchese G.F., Forte A.M., Pappagallo M.Letter to the Editor, 90 - 92 -
Meniscal sutures with outside-in technique: our experience with a less expensive method
Calderazzi F., Violi C., Paraskevopoulos A., Schiavi P., Ceccarelli F.Letter to the Editor, 93 - 94 -
No difference at two years between all inside transtibial technique and traditional transtibial technique in anterior cruciate ligament reconstruction
Volpi P., Balt C., Cervellin M., Denti M., Prospero E., Morenghi E., Quaglia A.Letter to the Editor, 95 - 99