| To determine if tendon cell ciliary length could be used as a biomarker of cytoskeletal tensional homeostasis, 20 mm long adult rat tail tendons were placed in double artery clamps set 18 mm apart to create a 10% laxity. The tendons were allowed to contract over a 7 day period under culture conditions. At 0, 1, 5, and 7 days the tendon cell cilia were stained and ciliary length measured using confocal imaging. There was a significant (p<0.001) increase in ciliary length at 1 day. At day 5 (when the tendon became visibly taut) there was a significant (p<0.001) decrease in ciliary length compared to day 1. By day 7 the tendon remained taut and ciliary length returned to day zero values (p=0.883). These results suggest that cilia length reflects the local mechanobiological environment of tendon cells and could be used as a potential in situ biomarker of altered cytoskeletal tensional homeostasis. |
Tendon cell ciliary length as a biomarker of in situ cytoskeletal tensional homeostasis
Lavagnino M., Gardner K., Sedlak A.M., Arnoczky S.P
Original Article, 118 - 121
Keywords: cilia, marker, recalibration, tendon, tensional homeostasis,
Table of Content: Vol. 3 (No. 3) 2013 July/September
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Sample size determination for clinical research
Wang D., Bakhai A., Del Buono A., Maffulli N.Original Article, 116 - 117 -
Tendon cell ciliary length as a biomarker of in situ cytoskeletal tensional homeostasis
Lavagnino M., Gardner K., Sedlak A.M., Arnoczky S.POriginal Article, 118 - 121 -
New insights in extracellular matrix remodeling and collagen turnover related pathways in cultured human tenocytes after ciprofloxacin administration
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