SUMMARY
Necroptosis is programmed cell death similar to necrosis, which is mediated by meticulous protein interactions such as those in apoptosis. Essentially, because it is a reaction
to inflammation, necroptosis functions similarly to apoptosis and it is thought that necroptosis is an alternative cell death pathway when apoptosis cannot function for some reason. However, the research history of necroptosis is brief, and although there are various studies, many aspects are unknown. Here, we found that calcium was an essential factor for necroptosis in a muscle cell line. After verifying necroptosis was functional in mouse skeletal muscle cell line C2C12, we found suppression of necroptosis by controlling the amount of calcium in the culture medium. Furthermore, the reduced necroptosis was rescued by adding calcium to the culture medium. However, the recovery did not occur when magnesium or sodium was added to the medium.
Next, we suppressed certain calcium transporters using inhibitors. Necroptosis was inhibited by reducing the amount of intracellular calcium. These results showed that necroptosis was controlled by the amount of calcium. This finding may facilitate development of new treatment methods for various muscle diseases that involve cell death.
Calcium is Involved in Necroptosis Through the Expression of Phospho-MLKL in Skeletal Muscle Cell in Mice
Ryu Yashiro, Yasunari Matsuzaka
Original Article, 211-217
Keywords: C2C12, calcium, necroptosis, p-MLKL, skeletal muscle,
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