SUMMARY
Background. Scaffolds composed of demineralized bone matrix (DBM) enriched with platelet rich plasma (PRP) and concentrated bone marrow-derived mesenchymal stem cells (concentrated bMSCs) may be able to release growth factors over time. The hypothesis is that the combination of PRP + bMSCs + DBM will result in high levels of growth factor release over prolonged periods of time.
Methods. Bone morrow (BM) and whole blood was purchased from Lonza (Lonza Inc, Allendale, NJ). Whole blood was processed to give PRP. BM was concentrated, cells were counted, plated, and grown to confluence. Cells were seeded onto the DBM and augmented with (+)/(-) PRP and concentrated bMSCs. Media were collected and analyzed for BMP-2, PDGF, TGF-β and MMP-2.
Results. bMSCs adhered to and proliferated on the DBM as well as tissue culture plastic (TCP). When PRP and concentrated bMSCs were both added to the DBM, there was significantly increased growth factor release of BMP-1, PDGF, and TGF-β. The negative control of MMP-2 showed no increased release throughout the time period measured.
Conclusions. Combining PRP and bMSCs with a DBM scaffold ensured consistently high levels of growth factor release over time. This may help to promote healing in-vivo, but remains to be proven in a clinical scenario.
Combining Platelet Rich Plasma and Concentrated Bone Marrow with a Demineralized Bone Matrix Scaffold Enhances Growth Factor Release Over Time
J. M. Brewer, M. B. McCarthy, L. Pauzenburger, M. P. Cote, A. D. Mazzocca
Original Article, 484-493
Keywords: concentrated bone marrow derived mesenchymal cells, demineralized bone matrix, growth factor release, platelet rich plasma,
Table of Content: Vol. 11 (No.3) 2021 July/September
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Postural Stability in Men with Anterior Cruciate and Bicruciate Ligament Knee Injury
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J. M. Brewer, M. B. McCarthy, L. Pauzenburger, M. P. Cote, A. D. MazzoccaOriginal Article, 484-493 -
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