SUMMARY
Background. Specific scaffolds are still required to sufficiently deliver and retain applied mesenchymal stem cells (MSCs) of various origins to the targeted zone of repaired tissue. The purpose of the study was to evaluate the demineralized bone matrix (DBM) as a scaffold for concentrated bone marrow aspirate (cBMA), subacromial bursa-derived cells (SBDCs), tenocytes, and osteoblasts.
Methods. cBMA was harvested from the proximal humerus along with tenocytes and subacromial bursal tissue during arthroscopic shoulder surgery. Human osteoblasts were obtained from femoral condyles of patients undergoing total knee arthroplasty. Cells were seeded on a DBM scaffold. Cellular adhesion and proliferation assays were performed for each scaffold sample. Half of cBMA underwent arthroscopic simulation of being washed to mimic the intraoperative environment.
Results. There was no significant difference in cellular adhesion potential on DBM scaffolds (p=0.168) when comparing SBDCs (mean±SD: 25,775±11,351), osteoblasts (mean±SD: 33,113±2,709), tenocytes (mean±SD: 25,000±3,864) and cBMA (mean±SD: 25,025±6,206). Osteoblasts (mean±SD: 0.279±0.009) showed a significantly higher proliferation potential (p=0.021, respectively) compared to SBDCs (mean±SD: 0.248±0.015), tenocytes (mean±SD: 0.239±0.011), and cBMA (mean±SD: 0.247±0.007). Histology sections and electron microscopy confirmed the presence of cells within the matrix of scaffolds.
Conclusions. SBDCs and cBMA demonstrated high cellular adhesion and proliferation potential on DBM scaffolds.
Concentrated Bone Marrow Aspirate and Subacromial Bursa-Derived Cells Demonstrate Similar Cellular Adhesion and Proliferation Potential on Demineralized Bone Matrix Scaffolds for Biologic Augmentation of Rotator Cuff Repair
L. N. Muench, D. P. Berthold, C. Kia, A. Lebaschi, M. P. Cote, M. B. McCarthy, A. D. Mazzocca
Original Article, 48-56
Keywords: Biologic augmentation, concentrated bone marrow aspirate, demineralized bone matrix, rotator cuff repair., scaffold, shoulder surgery., subacromial bursa,
Table of Content: Vol. 10 (No. 1) 2020 January/March
-
Thanks to the reviewers
Acknowledgments, 0 -
Chronic Distal Triceps Brachii Tendon Ruptures. A Systematic Review of Surgical Procedures and Outcomes
F. Oliva, F. F. Sesti, M. Gasparini, L. PanarellaReview, 1-10 -
Menstrual Cycle does not Affect the Mechanical Properties of Muscle and Tendon
S. Taş, D. AktaşOriginal Article, 11-16 -
Effect of Fascial Manipulation on Glenohumeral Internal Rotation Deficit in Overhead Athletes A Randomized Controlled Trial
Nelson P Mathew, Prabu Raja G, F. DavisOriginal Article, 17-23 -
The Effectiveness of Physiotherapy in Idiopathic or Primary Frozen Shoulder: a Systematic Review and Meta-Analysis
E. Cavalleri, A. Servadio, A. Berardi, M. Tofani, G. GaleotoReview, 24-39 -
Effectiveness of Radial Shockwave Therapy in Calcific and Non-Calcific Tendinopathy of the Shoulder: a Systematic Review and Meta-Analysis
A. Ammendolia, N. Marotta, A. Demeco, C. Marinaro, L. Moggio, M. Barletta, C. CostantinoReview, 40-47 -
Concentrated Bone Marrow Aspirate and Subacromial Bursa-Derived Cells Demonstrate Similar Cellular Adhesion and Proliferation Potential on Demineralized Bone Matrix Scaffolds for Biologic Augmentation of Rotator Cuff Repair
L. N. Muench, D. P. Berthold, C. Kia, A. Lebaschi, M. P. Cote, M. B. McCarthy, A. D. MazzoccaOriginal Article, 48-56 -
Research proposal of a new clinic model for the interpretation of Lateral Elbow Pain: is it time to change?
L. Di Filippo, D. Pennella, F. Maselli, P. ArrigoniOriginal Article, 57-66 -
Distal Thigh Compression Garment Improves Female Athlete Knee Control and Sports Movement Safety Perceptions
D. Boschert, J. Nyland, J. Matsuno, S. Pletz, W. PolioOriginal Article, 67-77 -
Bone-patellar Tendon-bone Versus Four Strands Hamstring Grafts for Anterior Cruciate Ligament Reconstruction
F. Migliorini, J. Eschweiler, M. Tingart, M. Niewiera, B. RathOriginal Article, 78-85 -
Investigation of the Contraction Ratio of Transversus Abdominis and Internal Oblique Muscles during Lumbopelvic Stability Test
P. Sitilertpisan, L. Joseph, A. Paungmali, U. Pirunsan , T. ChunchaiOriginal Article, 86-91 -
Effect of a 12-week Eccentric and Isometric Training in Achilles Tendinopathy on the Gastrocnemius Muscle: an Ultrasound Shear Wave Elastography Study
M. Gatz, M. Betsch, M. Tingart, R. Michalik, F. Migliorini, T. Dirrichs, C. Kuhl, V. QuackOriginal Article, 92-99 -
Quantitative Weight Bearing and non-weight Bearing Measures of Stiffness in the Achilles Tendon and Gastrocnemius Muscle
G. Ericson Morgan, R. Martin, H. Welch, K. MorrisOriginal Article, 100-110 -
Descriptive Study on Distalmost Perforating Peroneal Artery Emphasizing Its Role in Flap Surgeries
R. Rai, M. Tonse, A. R. Rai, P. M. Dass, J. P. JanardhananOriginal Article, 111-118 -
The Posterior Lateral Femoral Contusion Sign (Aamer Botchu Sign): an Ancillary Sign of MCL Injury
A. Iqbal, E. McLoughlin, D. Beale, SL. James , R. BotchuOriginal Article, 119-123 -
Topographic Localization of Nerve to Masseter Muscle to determine the Primary Nerve- Muscle Relation, an Aid to Facial Sculpting Techniques
A. Ganapathy, V. Kumar, V. Prathapamchandra, JP Jessy, N. Rani, S. Kaler, S. SinghOriginal Article, 124-129 -
Mid Term Effects of a Single Injection versus Three Injections of Hyaluronic Acid in Patients with Rhizarthrosis
D. Perugia, R. M. Lanzetti, M. Vetrano Santoboni, M. C. VulpianiOriginal Article, 130-137 -
Morphological and Topographic Anatomy of Sesamoid Bones of Human Thumb
R. Abisshek Balaji, Apoorva, V. V. Katti, M. M. Pai, B. V. Murlimanju, L. V. PrabhuOriginal Article, 138-143 -
Saving Resources in Accident Emergency: a Simple Device for Injuries to the Extensor Tendons of the Hand
A. Oliviero, G. Criscuolo, F. Oliva, N. MaffulliOriginal Article, 144-145 -
Traumatic Dislocation of Three Major Joints in the Lower Limb: Management and Outcome
G. Maccagnano, A. Notarnicola, G. Sammarco, L. Scialpi, B. MorettiCase Report, 146-150 -
Defensive Antibacterial Coating (DAC ® ) for prevention of infection in ACL reconstruction: a feasibility study
R. Aicale, F. Oliva, N. MaffulliOriginal Article, 151-153