SUMMARY
Background. The purpose of this study was to compare subacromial bursa to concentrated bone marrow aspirate (cBMA) in a murine, tendon-bone healing model. The authors hypothesized that subacromial bursa would show similar biomechanical, histological, and cell survivability to that of cBMA.
Methods. Thirty-nine NSG mice were included in the study. All mice underwent simulated patella tendon avulsion injuries and were divided into one of three treatment groups: subacromial bursa, cBMA, or fibrin clot only. Subacromial bursa and cBMA were obtained from a single patient during primary arthroscopic rotator cuff repair (male, age: 57 years). All mice were sacrificed at 2 weeks for biomechanical, histologic, and immunohistochemistry evaluation. Biomechanical analysis included load to failure and stiffness, with the contralateral knees used as controls. Human PCR analysis was performed to confirm survivability in the bursa and cBMA groups.
Results. With respect to the contralateral intact limb, treatment with subacromial bursa significantly increased load to failure (66% of intact) compared to fibrin only (47.87% of intact, P=0.018). cBMA was found to restore tendon strength to 49.9% to its intact side, however no significant difference was found when compared to bursa (P=0.06). No significant difference in stiffness was found between groups. Human nuclear antigen was positive in 75% of the bursa treated mice compared to only 25% of the cBMA specimens.
Conclusions. Subacromial bursa tissue demonstrated significant improvement in tendon strength compared to fibrin clot treatment (acellular control) at 2 weeks. Increased human cell survivability was also demonstrated using bursal tissue compared to cBMA.
Histological and Biomechanical Evaluation of Biologic Adjuvants in a Murine Tendon-Bone Healing Model
J. Johnson, C. Kia, D. Morikawa, J. Mehl, F.B. Imhoff, A. Otto, L.N. Muench, M.Wolf, J.B. Baldino, X. Xin, M. B. McCarthy, A.D. Mazzocca
Original Article, 494-505
Keywords: bone marrow aspirate, rotator cuff, shoulder, subacromial bursa,
Table of Content: Vol.9 (No.4) 2019 October/December
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The Effect of Different Suture Materials on Achilles Tendon Metabolism: A Preliminary in vivo Study of mRNA levels in Rabbits
S. Ergün, A. Alakbarov, A. Mine Yılmaz, B. Karademir, U. AkgünOriginal Article, 470-477 -
Electrospun Scaffolds in Tendons Regeneration: a review
R. Di Gesù, G. Amato, R. GottardiOriginal Article, 478-493 -
Histological and Biomechanical Evaluation of Biologic Adjuvants in a Murine Tendon-Bone Healing Model
J. Johnson, C. Kia, D. Morikawa, J. Mehl, F.B. Imhoff, A. Otto, L.N. Muench, M.Wolf, J.B. Baldino, X. Xin, M. B. McCarthy, A.D. MazzoccaOriginal Article, 494-505 -
Return to Sport After Patellar Tendon Rupture: a Systematic Review
J. Grondin, P. Menu, T. Garraud, O. Mesland, M. Dauty, A. Fouasson-ChaillouxOriginal Article, 517-524 -
Anti-inflammatory effects of two platelet-rich gel supernatants in an in vitro system of ligament desmitis
C. Castillo-Franz, C. López, M.E. Álvarez, C.E. Giraldo, J.U. CarmonaOriginal Article, 506-516 -
The Effect of Addition of Buffered Dextrose 5% Solution on Pain Occurring During Local Steroid Injection for Treatment of Plantar Fasciitis: A Randomized Controlled Trial
A. Moshrif, M. ElwanOriginal Article, 525-530 -
Patient-Reported Outcome and Healing Biomarkers in Patients Treated by Female versus Male Surgeons – A cohort study on Achilles tendon ruptures
J. Flodin, R. Juthberg, G. Edman, P. AckermannOriginal Article, 531-538 -
Motor imagery training decreases pain on loading in people with moderate Achilles tendinopathy: A preliminary randomised cross-over experiment
J. Debenham, S. Krummenacher, I. Skinner, D. Hince, M. Travers, B. WandOriginal Article, 539-543 -
Quantifying the Dimensions of Achilles Tendon Insertional Area Using Ultrasound Imaging A Validity and Reliability Study
N.H. Alghamdi, M. Killian, B. Aitha, R.T. Pohlig, K. Grävare SilbernagelOriginal Article, 544-551 -
Combined ESWT & RSW Therapy for Achilles Tendinopathy: A Prospective Study
A. Saxena, L. Shou, S. AshrafOriginal Article, 552-561 -
Dynamic Lisfranc Joint Repair Concept: Surgical Technique for a Synthetic Neoligamentplasty
C. Nery, E. Giza, E. Wagner, M. A. Percope de Andrade, T. Soares Baumfeld, M. Pires Prado, D. BaumfeldOriginal Article, 562-570 -
Superior Capsule Reconstruction for Irreparable Rotator Cuff Tear with a Porcine Dermal Graft: Preliminary Results at 2 Years Minimum Follow-up
R. Dukan, A. Bommier, M. A. Rousseau, P. BoyerOriginal Article, 571-578 -
Intermuscular Connections in Anterior Brachium: its Implications in Radial Nerve Entrapment Neuropathy
M. Tonse, M. M. Pai, L.V. Prabhu, B.V. Murlimanju, R. Vadgaonkar, Y.L. RaoOriginal Article, 579-583 -
Treatment of Lateral Epicondylitis with Collagen Injections: a Pilot Study
B. Corrado, G. Mazzuoccolo, L. Liguori, V. A. Chirico, M. Costanzo, I. Bonini, G. Bove, L. CurciOriginal Article, 584-589 -
Short-Term Efficacy and Safety of Betamethasone Valerate 2.25 mg Medicated Plaster in Patients with Chronic Lateral Epicondylitis: Results of a Randomised, Double Blind, Placebo-controlled Study
S. Bernasconi, A. Causero, G. Giaffreda, R. Papalia, G. Caruso, G. Bonanno, F. Sadile, G. Fraschini, M. Fortina, A. Frizziero, N. Chiarelli, V. Salini, U. Tarantino, N. Voglino, A. Castagna, F. A. Grassi, S. Rovati, V. FrangioneOriginal Article, 590-599 -
A diagnostic Approach to Deltoid Muscle Masses
A. Iqbal, E. McLoughlin, A. Patel, A.M. Davies, S.L. James, R. BotchuOriginal Article, 600-605 -
Efficacy of a Treatment for Gonarthrosis Based on the Sequential Intra-Articular Injection of Linear and Cross-Linked Hyaluronic Acids
E. Barbieri, I. Capparucci, F. Mannello, G. Annibalini, S. Contarelli, L. Vallorani, A. M. Gioacchini, D. Ligi, R. Maniscalco, M. Gervasi, T. Tran Dang Xuan, C. Bartolucci, V. Stocchi, P. SestiliOriginal Article, 606-614 -
The Effect of Segmental Stabilization Exercises on Pain, Disability and Static Postural Stability in Patients with Spondylolisthesis: A Double Blinded Pilot Randomized Controlled Trial
R. Choopani, F. Ghaderi, Z. Salahzadeh, H. Adigozali, H.N. Sivaki, E.M. Majd, M.R. Azghani, A. Soltani, M.A. JafarabadiOriginal Article, 615-626 -
Matside Medical Aid during Judo Competition in Japan
A. Ikumi, N. Sakuyama, N. Takatori, T. Kamitani, S. Miyazaki, Y. Nimura, N. Maffulli, N. G. Malliaropoulos, S. NagahiroOriginal Article, 627-634 -
Synergistic Effect of Physical Therapy Plus Pharmacological Therapy with Eperisone in Tension-Type Cervicalgia
C. Asaro, D. Scaturro, S. Tomasello, L.G. Tumminelli, G. Letizia MauroOriginal Article, 635-641