SUMMARY
Background. Autologous Platelet-Rich Plasma (PRP) therapy is used for treatment of tendinopathy. Efficacy, however, is variable, possibly caused by differences in PRP platelet concentrations. In an animal model of tendinopathy, we determined if there was an optimal PRP platelet concentration.
Methods. Tendinopathy was induced by collagenase injections into the Achilles tendons in rats. 10 days later, PRP at platelet concentrations of 50 (P50 group), 75 (P75 group), 100 (P100 group) and 150 ×104/µL (P150 group) or normal saline (control group) was injected into the tendons. To assess pain relief, spontaneous locomotor activity was measured for 12 hours at night. After 19 days, Achilles tendons were removed, and histological sections stained with hematoxylin-eosin or by TdT-mediated dUTP nick end labeling.
Results. Activities in the P75 and P100 groups were significantly greater than in the P50, P150 and control groups. The numbers of microtears, laminations and apoptotic cells in tendons in the P75 and P100 groups were significantly fewer than in the P50, P150 and control groups.
Conclusions. Pain relief and tendon repair were greatest in the P75 and P100 groups. In this rat model of tendinopathy, the optimal PRP platelet concentration in PRP was approximately a range of 75 to 100 ×104/µL.
Optimal Platelet Concentration for The Therapeutic Effect of Autologous Neutrophil-Reduced Platelet Rich Plasma in A Rat Model of Achilles Tendinopathy
M. Yoshida, H. Funasaki
Original Article, 612-621
Keywords: Optimal concentration of platelets; platelets-rich plasma; tendinopathy; animal model; enthesopathy; Achilles tendinitis.,
Table of Content: Vol. 10 (No.4) 2020 October/December
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