SUMMARY
Background. Tendon injuries are responsible for a crescent number of people retirement around the world. Recently, cell-based therapies has been shown to be effective for the treatment of tendon injures.
Objective. We aimed to evaluate the effect of local treatment with conditioned medium produced by tenocytes cell cultures on the histological and functional recovery in tenotomized mice.
Methods. The tendon cells were cultured for conditioning culture medium that will be used as treatment. The animals were subjected to right Aquilles tenotomy and treated with saline solution (SAL), DMEM (DMEM) and DMEM conditioned in tenocytes cultures (CM) and compared to the control group (CTRL). For histological analysis, tissues were stained with HE. Tendon functionality was measured using the Achilles Functional Index (AFI) and mechanical sensitivity through the paw withdrawal threshold (PWT) using the Von Frey test. Analyzes were performed at 7-, 14- and
21-days post-injury (dpi). Statistics were performed by ANOVA-2 followed by Tukey’s post-test, p < 0.01.
Results. In the histological analysis, the CM group showed better tissue organization when compared to SAL and DMEM groups. The CM group showed improvement in the PWT at 7° and 14° dpi (2.24 ± 1.15; 2.66 ± 1.06) compared to the DMEM (0.15 ± 0.07; 0 .45 ± 0.76 p < 0.01) and SAL (0.13 ± 1.15; 0.77 ± 0.95 p < 0.01). The CM group showed functional improvement at 7° and 14° dpi (-40.4 ± 12.6; -36.6 ± 10.4) compared to the DMEM (-76.5 ± 11.7; -71, 6 ± 7.9, p < 0.01) and SAL (-88.8 ± 15; -71.4 ± 12.6 p < 0.01).
Conclusions. We conclude that treatment with tenocytes conditioned medium accelerates tendon recovery, promoting improvement in mechanical sensitivity, functionality and tissue organization in the proposed injury model.
Treatment with Conditioned Medium from Tenocytes Primary Cell Cultures Accelerates Histological and Functional Recovery of Achilles Tendon in Tenotomized Mice
Analú Maciel, Milena Letícia Martins, Diego Rodrigues De Paula, Martha Souza França, Luana Ketlen Reis Leão, Suellen Alessandra Soares de Moraes, Rosivaldo dos Santos Borges, Evander de Jesus Oliveira Batista, Adelaide Conceição Fonseca Passos, Karen Renata Herculano Matos Oliveira, Anderson Manoel Herculano
Original Article, 5-10
Keywords: cell therapy, recovery., tendinopathy, tendon, tenocytes,
Table of Content: Vol. 14 (No.1) 2024 January/March
-
Leading by Example: A Call to Action for the New Committee “I.S.Mu.L.T. Sport and Disability” to Promote the Benefits of Sports for Individuals with Severe Disabilities
Augusto Fusco, Alessio Giai Via, Bernardo InnocentiEditorial, 2-4 -
Treatment with Conditioned Medium from Tenocytes Primary Cell Cultures Accelerates Histological and Functional Recovery of Achilles Tendon in Tenotomized Mice
Analú Maciel, Milena Letícia Martins, Diego Rodrigues De Paula, Martha Souza França, Luana Ketlen Reis Leão, Suellen Alessandra Soares de Moraes, Rosivaldo dos Santos Borges, Evander de Jesus Oliveira Batista, Adelaide Conceição Fonseca Passos, Karen Renata Herculano Matos Oliveira, Anderson Manoel HerculanoOriginal Article, 5-10 -
Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice
Matheus Felipe Zazula, Ana Paula Marca, Mylena de Campos Oliveira, Ariadne Barbosa, Maria Lúcia Bonfleur, Lucinéia de Fátima Chasko Ribeiro, Márcia Miranda TorrejaisOriginal Article, 12-21 -
Injuries Associated to Subtalar Dislocation: A Systematic Review
Alessandro Bartoli, Salvatore Ziello, Filippo Migliorini, Francesco OlivaReview, 22-28 -
Therapeutic Exercise in Plantar Fasciitis: A Systematic Review with Meta-Analysis
Renata Anddresa Stachelski, Beatriz Torrilhas, Franciele Foschiera Camboin, Dartel Ferrari de Lima, Cristiane Buzanello Donin, Gladson Ricardo Flor Bertolini, Márcia Rosângela BuzanelloReview, 29-45 -
Biomechanics of Meniscal Allograft Transplantation: A Meta-Analysis
Shayne Kelly, Kunal Panwar, Brenden Cutter, Nathan Reyes, Eric G. Huish JrReview, 46-53 -
Efficacy of Surgical Management versus Conservative Management to Decrease the Incidence of Re-Rupture in Adult Patients with Achilles Tendon Rupture: A Systematic Review and Meta-Analysis
Fátima María Guevara-Chávez, José Caballero-Alvarado, Carlos Zavaleta-CorveraReview, 54-66 -
Postural Control Pre- and Post-Group Supervised Clinic Based Exercise or Home Based Exercise in Patients with Knee Osteoarthritis: A Randomized Clinical Trial
Évelin Santos Vaz, Juliana Corrêa Soares, Carlos Bolli Mota, Michele Forgiarini SaccolOriginal Article, 67-74 -
Effects of Manual and Instrumental Therapy on the Treatment of Myofascial Pain Syndrome in Descending Trapezius of Women: A Blind Randomized Clinical Study
Vivian Carla Florianovicz, Bruna Scheffer Bueno, Ameg Dalpiaz, Heloyse Uliam Kuriki, Alexandre Márcio Marcolino, Fernando Diefenthaeler, Elaine Caldeira de Oliveira Guirro, Rafael Inácio BarbosaOriginal Article, 75-86 -
Effects of Ischemic Pre and Postconditioning on Indirect Markers of muscle damage: A Systematic Review and Meta-Analysis
Ingrid Martins de França, Mikhail Santos Cerqueira, Yves Matheus Barros de Sousa Oliveira, Stephen D. Patterson, Wouber Hérickson de Brito VieiraReview, 87-101 -
Lower Extremity Tendinopathies are Associated with Metabolic and Chronic Diseases: A Systematic Review
Iris F. Lagas, Branco K.J.F. Nijst, Fred Hartgens, Rob A. de Bie, Marijn Vis, Jan A.N. Verhaar, Robert-Jan de VosReview, 102-130 -
Bibliometric and Altimetric Analysis of Research Relating to Percutaneous Electrolysis
Bülent Alyanak, Mustafa Turgut Yıldızgören, Fatih BağcıerOriginal Article, 131-144 -
Effects of Subscapularis Muscle Dry Needling on clinical symptom improvement in People with Frozen Shoulder: A Randomized Controlled Trial Protocol
Amin Momenzadeh, Hakimeh Adigozali, Jalal Ahadi, Ali E. OskoueiOriginal Article, 145-155 -
Ultrasonography and Electromyography Activity of Abdominal Muscles in Different Abdominal Exercises: A Systematic Review
Helia Parvizi, Minoo Khalkhali Zavieh, Aliyeh Daryabor, Nahid TahanReview, 156-167 -
Peculiarities of Adolescent, Qualified Female Volleyball Players’ Shoulder Girdle
Krista Elīza Sakne, Agris Liepa, Inese PontagaOriginal Article, 168-179 -
Dual Task Reduces Balance Score but not Joint Repositioning Error in Female Athletes with Dynamic Knee Valgus
Fahime Naderi, Rahman Sheikhhoseini, Hashem Piri, Hamidreza ZanguieOriginal Article, 180-187 -
Neuromuscular Control Training is Effective to Prevent Ankle Sprains in Athletes
Homa Naderifar, Shima Babakhanian, Roya Najafi-Vosough, Mohammad Karimizadeh ArdakaniOriginal Article, 188-196 -
Reliability and Sensitivity of Smartphone Accelerometer to Assess Postural Balance in Healthy Individuals
José Carlos dos Santos Albarello, Conrado Torres Laett, Sidnei Cavalcante da Silva, Thiago LemosOriginal Article, 197-202 -
Combination of Training to Failure and Non-Failure: An Unexplored Approach of Number of Repetitions, Rate of Perceived Exertion, Time Under Tension, Volume-Load, and Muscle Swelling in Trained Individuals
Gustavo F. Pedrosa, Arthur B.G. Bischoff, Antony K. Luchese, Maria E. C. Rigo, Douglas G.D. Silva, Leonardo R. Marques, Tiago D. L. Nunes, Lorenzo I. Laporta, Fábio J. LanferdiniOriginal Article, 203-218 -
Flexor Tendon Repair with Fast-Absorbable Sutures: A Rupture Incidence-Focused Analysis of a Case Series and a Review
Valdas MacionisReview, 219-227 -
Multilevel Multiplane Two-Strand Technique for Flexor Tendon Repair: A Theoretical Proposal and Physical Considerations
Valdas MacionisOriginal Article, 228-229